Network inference from high throughput expression data

Posted on by Colin Semple

Suzuki et al (2009) examine the genome-wide dynamics of promoter usage in a human leukemia cell line, using high throughput sequencing of RNA over a time course of growth arrest and differentiation. Many key transcription regulators and their target genes were identified and validated by systematic siRNA knockdown. The results emphasise the enormous and daunting complexity of networks maintaining cellular states.